Amperometric biosensor for hydrogen peroxide and nitrite based on hemoglobin immobilized on one-dimensional gold nanoparticle

作者：Hong Jianmin; Dai Zhihui^*

来源：Sensors and Actuators B: Chemical , 2009, 140(1): 222-226.

DOI：10.1016/j.snb.2009.04.032

• Biosensors
• Hemoglobin
• One-dimensional gold nanoparticles
• Hydrogen peroxide
• Nitrite

Hemoglobin (Hb) was immobilized on one-dimensional (I D) gold (Au) nanoparticles to construct a novel amperometric biosensor for hydrogen peroxide (H(2)O(2)) and nitrite (NO(2)(-)). 1D Au nanoparticles were prepared by electrodepositon in the pores of an alumina template and were characterized by XRD and TEM. The immobilized Hb retained its biological activity well and displayed a good response to the reduction of both H(2)O(2) and NO(2)(-). Its apparent Michaelis-Menten constants for H(2)O(2) and NO(2)(-) were 10.8 and 35.3 mu M, respectively, showing a good affinity. Under optimal conditions, the biosensors could be used for the determination of H(2)O(2) ranging from 0.6 to 12.4 mu M and NO(2)(-) ranging from 0.4 to 14.8 mu M. The detection limits were 2.4 x 10(-8) and 6.5 x 10(-8) M at 3 sigma. respectively. 1D Au nanoparticles provided a good matrix for protein immobilization and biosensor preparation.

• 出版日期2009-6-18
• 单位南京大学; 南京师范大学