A new member of the ribbon-helix-helix transcription factor superfamily from the plant pathogen Xanthomonas axonopodis pv. citri

作者：Gallo Mariana; Ferrari Elena; Eliseo Tommaso; Amata Irene; Pertinhez Thelma A; Katsuyama Angela M; Paci Maurizio; Farah Chuck S; Spisni Alberto^*; Cicero Daniel O

来源：Journal of Structural Biology, 2010, 170(1): 21-31.

DOI：10.1016/j.jsb.2009.12.022

• Citrus canker
• Ribbon-helix-helix fold
• DNA-binding protein
• Antitoxin
• NMR

XACb0070 is an uncharacterized protein coded by the two large plasmids isolated from Xanthomonas axonopodis pv. cirri, the agent of citrus canker and responsible for important economical losses in citrus world production. XACb0070 presents sequence homology only with other hypothetical proteins belonging to plant pathogens, none of which have their structure determined. The NMR-derived solution structure reveals this protein is a homodimer in which each monomer presents two domains with different structural and dynamic properties: a folded N-terminal domain with beta alpha alpha topology which mediates dimerization and a long disordered C-terminal tail. The folded domain shows high structural similarity to the ribbon-helix-helix transcriptional repressors, a family of DNA-binding proteins of conserved 3D fold but low sequence homology: indeed XACb0070 binds DNA. Primary sequence and fold comparison of XACb0070 with other proteins of the ribbon-helix-helix family together with examination of the genes in the vicinity of xacb0070 suggest the protein might be the component of a toxin-antitoxin system.

• 出版日期2010-4