A Novel Biological Role of -Mangostin via TAK1-NF-B Pathway against Inflammatory

作者：Zou, Wenshu; Yin, Peng; Shi, Yaran; Jin, Na; Gao, Qian; Li, Jiandong; Liu, Fenghua^*

来源：Inflammation, 2019, 42(1): 103-112.

DOI：10.1007/s10753-018-0876-6

• -mangostin
• inflammatory
• LPS
• TAK1
• NF-B

The oxysterone -mangostin is isolated from mangosteen husks and is widely used in the treatment of abdominal pain, diarrhea, and dysentery. In this study, we established a lipopolysaccharide (LPS)-induced inflammatory model of rat intestinal epithelial cells (IEC-6 cells), at the same time we used differently concentration -mangostin to detect its anti-inflammatory activity. We applied doses of -mangostin (2.5, 5, and 10M) and detected apoptosis by flow cytometry, and the Griess reagent and the enzyme-linked immunosorbent assay (ELISA) method detected inflammatory factors such as nitric oxide (NO), prostaglandin (PG) E2, interleukin (IL)-1, IL-6, and tumor necrosis factor (TNF)-. We also used quantitative real-time PCR (Q-PCR) to examine inflammatory factors and western blotting to examine the activation of transforming growth factor-activated kinase (TAK)-1-nuclear factor (NF)-B signaling pathway-related proteins. Finally, we used laser confocal microscopy to detect the effect of the 10M -mangostin on the nuclear import of NF-B-p65. The results showed that -mangostin treatment significantly reduced the apoptosis of LPS-stimulated IEC-6 cells, the production of inflammatory factors, the activation of TAK1-NF-B signaling pathway-related proteins, and the entry of p65 into the nucleus. In conclusion, -mangostin exerts its anti-inflammatory effects by inhibiting the activation of TAK1-NF-B and it may be a potential choice for the treatment of inflammation diseases.

• 出版日期2019-2
• 单位中国科学院微生物研究所