Microinjection into cultured hippocampal neurons: A straightforward approach for controlled cellular delivery of nucleic acids, peptides and antibodies

作者：Lappe Siefke Corinna; Maas Christoph; Kneussel Matthias^*

来源：Journal of Neuroscience Methods, 2008, 175(1): 88-95.

DOI：10.1016/j.jneumeth.2008.08.004

• Microinjection
• Neuron
• RNAi
• Antisense oligonucleotide
• Antibody
• Fusion protein
• Peptide

Functional studies in neurons often require controllable simultaneous delivery of different molecules to individual cells within networks. Microinjection represents a suitable and alternative method to deliver cDNAs, oligonucleotides,siRNAs, peptides or antibodies for expression, expression knockdown or loss-of-function studies, respectively. Moreover, molecules can be systematically applied to individual neurons in a controlled manner without affecting neighbouring cells. Establishment of microinjection is often complicated and time consuming. Here we describe a simple and reliable protocol for molecular cell biologists to establish injection of various molecules (ng to mu g range) to living neurons in a reasonable period of time.

• 出版日期2008-10-30