A rapid ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometric method for the qualitative and quantitative analysis of the constituents of the flower of Trollius ledibouri Reichb.

作者：Li Xiaoqin; Xiong Zhili; Ying Xixiang; Cui Lanchong; Zhu Wenliang; Li Famei^*

来源：Analytica Chimica Acta, 2006, 580(2): 170-180.

DOI：10.1016/j.aca.2006.07.069

• ultra-performance liquid chromatography
• electrospray ionization tandem mass spectrometry
• qualitative and quantitative analysis
• flavonoids
• Trollius ledibouri Reichb.
• traditional Chinese medicine

A rapid ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometric (UPLC-ESI-MS/MS) method was developed for the qualitative and quantitative determination of the constituents of the flower of Trollius ledibouri Reichb. The analysis was performed on an AcQuity UPLC (TM) BEH C-18 column using gradient elution with a mobile phase of 0.1% acetic acid and acetonitrile over 20 min. A tandem quadrupole spectrometer operating in either full scan mode or in MS/MS mode for multiple reaction monitoring (MRM) was used for the qualitative and quantitative analysis of the constituents, respectively. According to the mass spectrometric fragmentation mechanism and UPLC-ESI-MS/MS data, the chemical structures of 15 constituents of the flower of T ledibouri Reichb. were identified on-line without time-consuming isolation and four of them, 2"-O-beta-L-galactopyranosylorientin, 2"-O-beta-arabinopyranosylorientin, orientin and vitexin, were quantified. The limits of quantification of these four flavonoids were 540, 321, 515 and 220 mu g g(-1) plant material, respectively. Four commercial samples from different sources were analyzed. The UPLC-ESI-MS/MS method for analyzing the constituents can be used to evaluate the quality of the flower of T. ledibouri Reichb.

• 出版日期2006-11-24
• 单位沈阳药科大学