Detection of Superoxide Anion Radical in a Stratum Corneum Intercellular Lipid Model Using an Electrochemical Sensor

作者：Kaneko Teruhisa; Kaise Chihiro; Kimoto Yoko; Suzuki Shota; Kondo Takeshi; Yuasa Makoto^*

来源：Journal of Oleo Science, 2011, 60(12): 647-654.

DOI：10.5650/jos.60.647

• stratum corneum intercellular lipid
• reactive oxygen species (ROS)
• superoxide anion radical (O-2(-)center dot)
• electrochemical sensor
• thiobarbituric acid (TBA) method

A stratum corneum intercellular lipid model was prepared in a quasi-non-aqueous system. It was found that the detection of the superoxide anion radical (O-2(-)center dot) generated in the lipid model by ultraviolet (UV) irradiation was possible using an electrochemical O-2(-)center dot sensor. The use of an electron spin resonance spin trap method confirmed that the reactive oxygen species generated in the lipid model by UV irradiation was O-2(-)center dot; the presence of a hydroxyl radical (center dot OH) was also proven. In addition, a reduction in the electric current in the O-2(-)center dot sensor was observed in lipid models containing added antioxidants such as d-alpha-tocopherol and beta-carotene. Moreover, there was a correlation between the degree of oxidative degradation of the lipid, which was determined by the thiobarbituric acid method, and the electric current due to the O-2(-)center dot detected using the O-2(-)center dot sensor.

• 出版日期2011-12