An analysis of the concentration change of intermediate metabolites by gene manipulation in fatty acid biosynthesis

作者：Park Seo Young; Jeon Eunyoung; Lee Sunhee; Lee Jinwon; Lee Taiyong; Won Jong In^*

来源：Enzyme and Microbial Technology, 2012, 51(2): 95-99.

DOI：10.1016/j.enzmictec.2012.04.006

• Biodiesel
• Fatty acid biosynthesis
• Intermediate metabolites
• Acetyl-CoA
• Acetyl-CoA carboxylase
• Malonyl-CoA
• Malonyl-CoA:ACP transacylase

In this report, concentration of malonic acid and acetic acid produced in Escherichia coli were investigated by the expression of acetyl-CoA carboxylase genes (accs) and a malonyl-CoA:ACP transacylase gene (fabD). Both malonyl-CoA and acetyl-CoA are essential intermediate metabolites in the fatty acid biosynthetic pathway, and are reversibly transformed to malonic acid and acetic acid, respectively in the cell. Acetyl-CoA is converted to malonic-CoA by acetyl-CoA carboxylases (Accs), which are composed of 3 different subunits (AccA, AccB, and AccC), and the resulting malonyl-CoA is then converted to malonyl-[acp] by malonyl-CoA:ACP transacylase (FabD). In this study, these genes were separately cloned, and the influences of overexpression of 4 different genes on the concentration of malonic acid and acetic acid were analyzed. Compared with the wild type E. coli, a recombinant strain containing 3 acc genes together showed a 41.03% enhanced malonic acid production, and a 4.29-fold increased ratio of malonic acid to acetic acid.

• 出版日期2012-7-15