Antibody Recognition Force Microscopy Shows that Outer Membrane Cytochromes OmcA and MtrC Are Expressed on the Exterior Surface of Shewanella oneidensis MR-1

作者:Lower Brian H*; Yongsunthon Ruchirej; Shi Liang; Wildling Linda; Gruber Hermann J; Wigginton Nicholas S; Reardon Catherine L; Pinchuk Grigoriy E; Droubay Timothy C; Boily Jean Francois; Lower Steven K
来源:Applied and Environmental Microbiology, 2009, 75(9): 2931-2935.
DOI:10.1128/AEM.02108-08

摘要

Antibody recognition force microscopy showed that OmcA and MtrC are expressed on the exterior surface of living Shewanella oneidensis MR-1 cells when Fe(III), including solid-phase hematite (Fe(2)O(3)), was the terminal electron acceptor. OmcA was localized to the interface between the cell and mineral. MtrC displayed a more uniform distribution across the cell surface. Both cytochromes were associated with an extracellular polymeric substance.

  • 出版日期2009-5-1