Molecular Characterization of the Vitellogenin Receptor in Bactericera cockerelli (Sulc)

作者：Karina Villanueva Segura O; Favela Lara Susana; Lopez Monroy Beatriz; Ponce Garcia Gustavo; Rodriguez Sanchez Iram P; Quiroz Martinez Humberto; Refugio Lomeli Flores J; Flores Adriana E^*

来源：Southwestern Entomologist, 2016, 41(3): 605-612.

DOI：10.3958/059.041.0303

Potato purple top and zebra chip diseases are transmitted by potato psyllid, Bactericera cockerelli (Sulc), to tomato, Solanum lycopersicum L., and potato, Solanum tuberosum L., crops. The aim of this study was to characterize and quantify vitellogenin receptor in potato psyllid. We obtained partial characterization of the receptor by end-point PCR and sequencing using capillary electrophoresis. Relative quantification by real-time PCR was used to determine expression levels of the receptor in females and males. Similarity in vitellogenin sequence obtained in a laboratory was 89% compared with the sequence of the entire transcriptome of the species reported in GenBank. Expression analysis showed the vitellogenin receptor significantly expressed in potato psyllid females compared with males from potato plants. Further study of the vitellogenin receptor of potato psyllid is needed to determine its complete sequence and potential target to control the pest.

• 出版日期2016-9