Microtubule nucleation from a functionalised SiO2 EM grid

作者:Saha Abhijit*; Chakraborty Indrani; Kraft Christian; Bhushan Shashi; Ghosh Surajit
来源:RSC Advances, 2013, 3(21): 7688-7691.
DOI:10.1039/c3ra40273h

摘要

A novel Tris-NTA functionalised SiO2 EM grid is generated through covalent chemical functionalisation. Here, we show immobilisation of functional oligohistidine-tagged proteins and nucleation of microtubules from microtubule polymerase immobilised on the SiO2 EM grid surface.

  • 出版日期2013