A high-throughput in vitro model of human embryo attachment

作者：Ho Huiting; Singh Harmeet; Aljofan Mohamad; Nie Guiying^*

来源：Fertility and Sterility, 2012, 97(4): 974-978.

DOI：10.1016/j.fertnstert.2012.01.116

• Embryo attachment
• endometrial epithelial cells
• implantation
• trophoblast spheroids

Objective: To develop a simple, high-throughput and widely applicable in vitro human implantation model that assesses trophoblast spheroid attachment to human uterine epithelial cells. %26lt;br%26gt;Design: Experimental study to establish and validate the model. %26lt;br%26gt;Setting: Cell culture. %26lt;br%26gt;Cell(s): Lines BeWo, RL95-2, and AN3-CA. %26lt;br%26gt;Intervention(s): Labeling trophoblast spheroids with green fluorescence, selecting spheroids of size similar to implanting blastocysts by use of cell strainers, and assessing spheroid attachment by use of an automated microplate reader. %26lt;br%26gt;Main Outcome Measure(s): Establishment of a simple, reliable, and high-throughput in vitro model to study human embryo implantation. %26lt;br%26gt;Result(s): The assay enabled rapid fluorometric assessment of spheroid attachment to human endometrial epithelial cells (RL95-2 and AN3-CA) under different experimental conditions. The high-throughput assay was confirmed by conventional counting method to be highly reproducible and accurate. %26lt;br%26gt;Conclusion(s): The described methodology provides for the first time a high-throughput assay for the study of human embryo implantation and a promising tool for screening potential inhibitors or enhancers of embryo attachment. (Fertil Steril((R)) 2012; 97: 974-8.

• 出版日期2012-4
• 单位河北医科大学