An Efficient Method to Obtain Dedifferentiated Fat Cells

作者：Taniguchi Hiroaki; Kazama Tomohiko; Hagikura Kazuhiro; Yamamoto Chii; Kazama Minako; Nagaoka Yuki; Matsumoto Taro^*

来源：Jove-Journal of Visualized Experiments, 2016, (113): e54177.

DOI：10.3791/54177

• Developmental Biology
• Issue 113
• dedifferentiation
• fat cells
• ceiling culture
• pluripotency
• medium
• tissue engineering

Tissue engineering and cell therapy hold great promise clinically. In this regard, multipotent cells, such as mesenchymal stem cells (MSCs), may be used therapeutically, in the near future, to restore function to damaged organs. Nevertheless, several technical issues, including the highly invasive procedure of isolating MSCs and the inefficiency surrounding their amplification, currently hamper the potential clinical use of these therapeutic modalities. Herein, we introduce a highly efficient method for the generation of dedifferentiated fat cells (DFAT), MSC-like cells. Interestingly, DFAT cells can be differentiated into several cell types including adipogenic, osteogenic, and chondrogenic cells. Although other groups have previously presented various methods for generating DFAT cells from mature adipose tissue, our method allows us to produce DFAT cells more efficiently. In this regard, we demonstrate that DFAT culture medium (DCM), supplemented with 20% FBS, is more effective in generating DFAT cells than DMEM, supplemented with 20% FBS. Additionally, the DFAT cells produced by our cell culture method can be redifferentiated into several tissue types. As such, a very interesting and useful model for the study of tissue dedifferentiation is presented.

• 出版日期2016-7