A yeast two-hybrid assay reveals CMYA1 interacting proteins

作者：Xin, Xiangbo; Wang, Ting; Liu, Xinfeng; Sui, Guoning; Jin, Congfei; Yue, Yingwei; Yang, Shuping; Guo, Hong^*

来源：Comptes Rendus Biologies, 2017, 340(6-7): 314-323.

DOI：10.1016/j.crvi.2017.06.003

• CMYA1
• Yeast two-hybrid
• Screen
• Protein interaction
• Muscle development

CMYA1 is a protein that plays an important role in muscle development and contains a highly conserved Xin repeats region. However, the function of the Xin-repeats in CMYA1 is unknown, and there is little information regarding proteins that interact with the CMYA1/Xin-repeats in the bovine system. In this study, we generated a high-quality bovine muscle cDNA library and performed a yeast two-hybrid screen twice using both CMYA1 and the Xin-repeats as bait. There were 27 candidate-interacting proteins identified in this screen. Three of the 27 proteins (RPL35A, RPL21 and EIF3G) interacted with both CMYA1 and the Xin-repeats, and this interaction was further confirmed using one-to-one Y2H mating. These results showed that the three candidate proteins interacted with CMYA1/Xin-repeats and indicated that the Xin-repeats is a key region of CMYA1 required for protein interaction. In conclusion, our results provide new targets on the bovine CMYA1/Xin-repeats interacting proteins, and these findings provide an important reference for the study of how bovine muscle development is regulated.

• 出版日期2017-7
• 单位天津农学院