Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA

作者：Heussner Alexandra H; Auslaender Simon; Dietrich Daniel R^*

来源：Toxins, 2010, 2(6): 1582-1594.

DOI：10.3390/toxins2061582

• mycotoxin
• ochratoxin B
• monoclonal antibody
• ELISA
• western analysis

A monoclonal antibody specific to ochratoxin B (OTB) was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit of detection (LOD) for OTB of 3 mu g/L (8 nM), a limit of quantification (LOQ) of 3.7 mu g/L (10 nM), and a 50% inhibitory concentration (IC50) of 150 nM. Due to very low cross-reactivity to OTA (2.7%) and structurally related molecules (0%), this OTB-ELISA was found to be suitable to detect OTB with excellent precision in different matrices, i.e., beer, coffee and wine. Therefore, this OTB-ELISA will allow screening of OTB in food and feed products.

• 出版日期2010-6