Abscisic acid dynamics in roots detected with genetically encoded FRET sensors

作者：Jones Alexander M^*; Danielson Jonas A H; ManojKumar Shruti N; Lanquar Viviane; Grossmann Guido; Frommer Wolf B

来源：eLife, 2014, 3: e01741.

DOI：10.7554/eLife.01741

Cytosolic hormone levels must be tightly controlled at the level of influx, efflux, synthesis, degradation and compartmentation. To determine ABA dynamics at the single cell level, FRET sensors (ABACUS) covering a range similar to 0.2-800 mu M were engineered using structure-guided design and a high-throughput screening platform. When expressed in yeast, ABACUS1 detected concentrative ABA uptake mediated by the AIT1/NRT1.2 transporter. Arabidopsis roots expressing ABACUS1-2 mu (K-d similar to 2 mu M) and ABACUS1-80 mu (K-d similar to 80 mu M) respond to perfusion with ABA in a concentration-dependent manner. The properties of the observed ABA accumulation in roots appear incompatible with the activity of known ABA transporters (AIT1, ABCG40). ABACUS reveals effects of external ABA on homeostasis, that is, ABA-triggered induction of ABA degradation, modification, or compartmentation. ABACUS can be used to study ABA responses in mutants and quantitatively monitor ABA translocation and regulation, and identify missing components. The sensor screening platform promises to enable rapid fine-tuning of the ABA sensors and engineering of plant and animal hormone sensors to advance our understanding of hormone signaling.

• 出版日期2014-4-15