Application of Gel Permeation HPLC for Lipoprotein Profiling in Dogs

作者：Mizutani Hisashi^*; Sako Toshinori; Arai Noriaki; Kuriyama Koji; Yoshimura Itaru; Mori Akihiro; Iwase Kenji; Hirose Hisashi

来源：Journal of Veterinary Medical Science, 2010, 72(6): 813-817.

DOI：10.1292/jvms.09-0500

• canine
• cholesterol
• HPLC
• lipoprotein profile
• triglyceride

A high performance liquid chromatography system with a gel permeation column (GP-HPLC) and anon-line dual enzymatic system was applied to lipoprotein analysis in dogs. A high density lipoprotein (HDL) fraction obtained by conventional ultracentrifugation gave a single peak at around 28-29 min. Similarly, a low density lipoprotein (LDL) fraction gave single peak at around 24-25 min. The lipoprotein profiles of healthy dogs were contained large HDL peaks and small LDL peaks, and VLDL and CM were only marginally detected. In diabetic dogs, concentrations of VLDL-triglyceride and VLDL-total-cholesterol were elevated significantly. The lipoprotein profile analysis by GP-HPLC method would be useful in explication of abnormality of lipid metabolism in dogs.

• 出版日期2010-6