Antenna-predominant and male-biased CSP19 of Sesamia inferens is able to bind the female sex pheromones and host plant volatiles

作者：Zhang, Ya-Nan; Ye, Zhan-Feng; Yang, Ke; Dong, Shuang-Lin^*

来源：Gene, 2014, 536(2): 279-286.

DOI：10.1016/j.gene.2013.12.011

• The purple stem borer
• Chemosensory protein
• Expression pattern
• Prokaryotic expression
• Ligand-binding

Insect chemosensory proteins (CSPs) are proposed to capture and transport hydrophobic chemicals across the sensillum lymph to olfactory receptors (ORs), but this has not been clarified in moths. In this study, we built on our previously reported segment sequence work and cloned the full length CSP19 gene (SinfCSP19) from the antennae of Sesamia inferens by using rapid amplification of cDNA ends. Quantitative real time-PCR (qPCR) assays indicated that the gene was expressed in a unique profile, i.e. predominant in antennae and significantly higher in male than in female. To explore the function, recombinant SinfCSP19 was expressed in Escherichia coli cells and purified by Ni-ion affinity chromatography. Binding affinities of the recombinant SinfCSP19 with 39 plant volatiles, 3 sex pheromone components and 10 pheromone analogs were measured using fluorescent competitive binding assays. The results showed that 6 plant volatiles displayed high binding affinities to SinfCSP19 (Ki = 2.12-8.75 mu M), and more interesting, the 3 sex pheromone components and analogs showed even higher binding to SinfCSP19 (Ki =. 0.49-1.78 mu M). Those results suggest that S1nfCSP19 plays a role in reception of female sex pheromones of S. inferens and host plant volatiles.

• 出版日期2014-2-25
• 单位南京农业大学