A role for zinc in regulating hypoxia-induced contractile events in pulmonary endothelium

作者：Bernal, Paula J.; Bauer, Eileen M.; Cao, Rong; Maniar, Salony; Mosher, Mackenzie; Chen, Jun; Wang, Qiming Jane; Glorioso, Joseph C.; Pitt, Bruce R.; Watkins, Simon C.; St Croix, Claudette M.^*

来源：American Journal of Physiology - Lung Cellular and Molecular Physiology, 2011, 300(6): L874-L886.

DOI：10.1152/ajplung.00328.2010

• pulmonary circulation
• endothelial cells
• actin stress fibers

Bernal PJ, Bauer EM, Cao R, Maniar S, Mosher M, Chen J, Wang QJ, Glorioso JC, Pitt BR, Watkins SC, St. Croix CM. A role for zinc in regulating hypoxia-induced contractile events in pulmonary endothelium. Am J Physiol Lung Cell Mol Physiol 300: L874-L886, 2011. First published March 4, 2011; doi:10.1152/ajplung.00328.2010.-We previously reported that zinc thiolate signaling contributes to hypoxic contraction of small, nonmuscularized arteries of the lung. The present studies were designed to investigate mechanisms by which hypoxia-released zinc induces contraction in isolated pulmonary endothelial cells and to delineate the signaling pathways involved in zinc-mediated changes in the actin cytoskeleton. We used fluorescence-based imaging to show that hypoxia induced time-dependent increases in actin stress fibers that were reversed by the zinc chelator, N,N,N',N'-tetrakis-(2-pyridylmethyl)-ethylenediamine (TPEN). We further showed that hypoxia-induced phosphorylation of the contractile protein myosin light chain (MLC) and assembly of actin stress fibers were each TPEN sensitive. Hypoxia and zinc-induced inhibition of MLC phosphatase (MLCP) were independent of the regulatory subunit (MYPT1) of MLCP, and therefore hypoxia-released zinc likely inhibits MLCP at its catalytic (PP1) subunit. Inhibition of PKC by Ro-31-8220 and a dominant-negative construct of PKC-epsilon attenuated hypoxia-induced contraction of isolated pulmonary endothelial cells. Furthermore, zinc-induced phosphorylation of MLC (secondary to inhibition of MLCP) was PKC dependent, and hypoxia-released zinc promoted the phosphorylation of the PKC substrate, CPI-17. Collectively, these data suggest a link between hypoxia, elevations in labile zinc, and activation of PKC, which in turn acts through CPI-17 to inhibit MLCP activity and promote MLC phosphorylation, ultimately inducing stress fiber formation and endothelial cell contraction.

• 出版日期2011-6