Partially phosphonated polyethylenimine-coated nanoparticles as convenient support for enzyme immobilization in bioprocessing

作者：Monteil Clement; Bar Nathalie; Retoux Richard; Henry Joel; Bernay Benoit; Villemin Didier^*

来源：Sensors and Actuators B: Chemical , 2014, 192: 269-274.

DOI：10.1016/j.snb.2013.09.096

• Trypsin
• Phosphonated polyethylenimine
• Magnetic nanoparticles
• Enzyme immobilization

Partially phosphonated polyethylenimine (PEIP) has been developed as an easily functionalisable coating agent for iron oxide nanoparticles. Trypsin immobilization takes special advantage of the properties of this new material. Numerous enzymes can be loaded on the polymer by a covalent bounding with numerous amino groups. The PEIP contributes to the high stability of the material, through a strong covalent P-O-Fe bond. Resistance to hydrolysis and to temperature increasing ensure to obtain a highly recyclable magnetic nanomaterial designed for proteomic analysis.

• 出版日期2014-3