Multiplex PCR for Identification of Two Capsular Types in Epidemic KPC-Producing Klebsiella pneumoniae Sequence Type 258 Strains

作者：Chen Liang; Chavda Kalyan D; Findlay Jacqueline; Peirano Gisele; Hopkins Katie; Pitout Johann D D; Bonomo Robert A; Woodford Neil; Deleo Frank R; Kreiswirth Barry N^*

来源：Antimicrobial Agents and Chemotherapy, 2014, 58(7): 4196-4199.

DOI：10.1128/AAC.02673-14

We developed a multiplex PCR assay capable of identifying two capsular polysaccharide synthesis sequence types (sequence type 258 [ST258] cps-1 and cps-2) in epidemic Klebsiella pneumoniae ST258 strains. The assay performed with excellent sensitivity (100%) and specificity (100%) for identifying cps types in 60 ST258 K. pneumoniae sequenced isolates. The screening of 419 ST258 clonal isolates revealed a significant association between cps type and K. pneumoniae carbapenemase (KPC) variant: cps-1 is largely associated with KPC-2, while cps-2 is primarily associated with KPC-3.

• 出版日期2014-7
• 单位NIH; rutgers