Down-regulation of AQP8 suppresses glioma cells growth and invasion/migration via cell cycle pathway

作者：Dong, Rui; Tao, Shengzhong^*; Liu, Zhan; Zheng, Wei; Yu, Dan

来源：International Journal of Clinical and Experimental Pathology, 2016, 9(2): 1240-1248.

• Aquaporin8
• glioma cells
• proliferation
• invasion
• cell cycle

Aquaporin8 (AQP8), has a pivotal role in keeping the fluid and electrolyte balance, which is weakly distributed in mammalian brains. Overexpression of AQP8 has been reported in several types of human cancer but roles in human glioma have yet to be clearly defined. Here, we investigated the expression changes of AQP8 in 35 cases of human brain glioma tumors using Real-time PCR, and then down-regulated expression of AQP8 gene in human glioma U373 and T98G cells using specific targeted short hairpin RNA (shRNA). Its specific functions and mechanisms in human glioma cells were investigated as a potential therapeutic target for glioma. In this study, we found that AQP8 expression level was up-regulated in gliomas than normal brain tissues. Down-regulation of AQP8 in U373 and T98G cells showed a significant inhibitory effect on cell proliferation and invasion/migration, which accompanied with cell cycle S arrest as well as down-regulating the cell cycle protein expression. In summary, this study suggested that AQP8 suppresses glioma cells growth and invasion/migration via cell cycle pathway, and may be useful for developing a new therapeutic strategy for glioma.

• 出版日期2016
• 单位郑州大学