A prospective evaluation of diagnostic methodologies for the acute diagnosis of dengue virus infection on the Thailand-Myanmar border

作者：Watthanaworawit Wanitda^*; Turner Paul; Turner Claudia L; Tanganuchitcharnchai Ampai; Jarman Richard G; Blacksell Stuart D; Nosten Francois H

来源：Transactions of the Royal Society of Tropical Medicine and Hygiene, 2011, 105(1): 32-37.

DOI：10.1016/j.trstmh.2010.09.007

• Dengue
• PCR
• Serology
• Evaluation
• Diagnosis
• Thailand

Clinically useful diagnostic tests of dengue virus infection are lacking. We prospectively evaluated the performance of real-time reverse transcriptase (rRT)-PCR, NS-1 antigen and IgM antibody tests to confirm dengue virus infection in acute blood specimens from 162 patients presenting with undifferentiated febrile illness compatible with dengue infection. rRT-PCR was the most sensitive test (89%) and potentially could be used as a single test for confirmation of dengue infection. NS-1 antigen and IgM antibody were not sufficiently sensitive to be used as a single confirmatory test with sensitivities of 54% and 17% respectively. The specificities of rRT-PCR, NS-1 antigen and IgM antibody tests were 96%, 100% and 88% respectively. Combining NS-1 and rRT-PCR or the combination of all three tests resulted in the highest sensitivity (93%) but specificities dropped to 96% and 83% respectively. We conclude that at least the combination of two tests, either agent detection (rRT-PCR) or antigen detection (NS-1) plus IgM antibody detection should be used for laboratory confirmation of dengue infection.

• 出版日期2011-1
• 单位河北医科大学