A novel serine alkaline protease from Bacillus altitudinis GVC11 and its application as a dehairing agent

作者：Kumar E Vijay; Srijana M; Kumar K Kiran; Harikrishna N; Reddy Gopal^*

来源：Bioprocess and Biosystems Engineering, 2011, 34(4): 403-409.

DOI：10.1007/s00449-010-0483-x

• Alkaline protease
• Dehairing
• Bacillus
• Purification
• Fermentation

A serine alkaline protease from a newly isolated alkaliphilic Bacillus altitudinis GVC11 was purified and characterized. The enzyme was purified to homogeneity by acetone precipitation, DEAE-cellulose anion exchange chromatography with 7.03-fold increase in specific activity and 15.25% recovery. The molecular weight of alkaline protease was estimated to be 28 kDa by SDS PAGE and activity was further assessed by zymogram analysis. The enzyme was highly active over a wide range of pH 8.5 to 12.5 with an optimum pH of 9.5. The optimum temperature of purified enzyme was 45 A degrees C and Ca(2+) further increased the thermal stability of the enzyme. The enzyme activity was enhanced by Ca(2+) and Mg(2+) and inhibited by Hg(2+). The present study is the first report to examine and describe production of highly alkaline protease from Bacillus altitudinis and also its remarkable dehairing ability of goat hide in 18 h without disturbing the collagen and hair integrity.

• 出版日期2011-5