Membrane insertion of-and membrane potential sensing by-semiconductor voltage nanosensors: Feasibility demonstration

作者：Park, Kyoungwon; Kuo, Yung; Shvadchak, Volodymyr; Ingargiola, Antonino; Dai, Xinghong; Hsiung, Lawrence; Kim, Wookyeom; Zhou, Hong; Zou, Peng; Levine, Alex J.; Li, Jack; Weiss, Shimon^*

来源：Science Advances, 2018, 4(1): e1601453.

DOI：10.1126/sciadv.1601453

• QUANTUM DOTS
• LIVE CELLS
• CDSE NANOCRYSTALS
• IN-VITRO
• PEPTIDE
• NANOPARTICLES
• VESICLES
• BILAYER
• HETEROSTRUCTURES
• RECOMBINATION

We developed membrane voltage nanosensors that are based on inorganic semiconductor nanoparticles. We provide here a feasibility study for their utilization. We use a rationally designed peptide to functionalize the nanosensors, imparting them with the ability to self-insert into a lipid membrane with a desired orientation. Once inserted, these nanosensors could sense membrane potential via the quantum confined Stark effect, with a single-particle sensitivity. With further improvements, these nanosensors could potentially be used for simultaneous recording of action potentials from multiple neurons in a large field of view over a long duration and for recording electrical signals on the nanoscale, such as across one synapse.

• 出版日期2018-1
• 单位北京大学