科研之友
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摘要
In this study, a fast and simple analytical method for determination of urinary 18 beta-glycyrrhetinic acid (18-GA), without any sample purification prior to the analysis, was developed. The method was successfully applied to determine the 18-GA concentration in real urine samples obtained after ingestion of glycyrrhizin. The method was shown to be specific, accurate, linear over the tested range (correlation coefficients 0.999), and repeatable (RSD below 0.2 and 3.0% for retention time and peak area, respectively). A new multi-variable-at-a-time (MVAT) approach for robustness testing gives RSD values of 0.6 and 3.8% at "-1" level, and 4.7 and 12.3% at "+1" level compared to the optimal "zero" level for retention time and peak area, respectively.
- 出版日期2010-5
