Potential Applications of Solid Waste Generated in the Leather Industry as a Novel Supporting Matrix for Immobilization of Enzyme

作者：Hu Yang; Liu Lan; Dan Weihua; Dan Nianhua^*

来源：Journal of the Society of Leather Technologists and Chemists, 2014, 98(3): 121-126.

A novel method for the immobilization of an enzyme was established by using solid waste from tanneries as the carrier. A series of inorganic and organic carriers with good behaviours were evaluated in this context. The results showed that the enzyme immobilization efficiency was optimal when the enzyme offer, reaction temperature, pH, ionic strength and time were set at 335mg enzyme per g carrier, 25 degrees C, 6.5, 0.1mol/L and 60 minutes, respectively. The optimal temperature and pH of the immobilized enzyme in terms of protease were 55 degrees C and 8.0, respectively, similar to that for the free enzyme. The immobilized enzyme remained 64% of its original activity after keeping at 55 degrees C for 3 hours, while the free enzyme lost nearly 54% under the same conditions, indicating the improvement of enzyme thermal stability after enzyme immobilization. Furthermore, the leather industry oriented application of the immobilized enzyme was intended for the dehairing of cattle hide.

• 出版日期2014-6
• 单位四川大学