Difference in microchip electrophoretic mobility between partially and fully PEGylated poly(amidoamine) dendrimers

作者：Park Eun Ji; Na Dong Hee

来源：Analytical Biochemistry, 2015, 488: 9-11.

DOI：10.1016/j.ab.2015.07.016

• Microchip capillary gel electrophoresis
• PEGylation
• Poly(amidoamine) dendrimers

The objective of this study was to investigate the difference in electrophoretic mobility between partially and fully poly(ethylene glycol)-conjugated poly(amidoamine) dendrimers (part-PEG-PAMAM and full-PEG-PAMAM, respectively) using a microchip capillary gel electrophoresis (MCGE). While MCGE allowed size-based separation of PEG-PAMAMs prepared with monomethoxy PEG-nitrophenyl carbonate, full-PEG-PAMAMs migrated slower than part-PEG-PAMAMs that were similar in size or larger. When the measured molecular weights obtained from MCGE analysis and the calculated molecular weights were plotted, each part-PEG-PAMAM and full-PEG-PAMAM showed correlation coefficients greater than 0.98. This study indicates that MCGE would be useful for characterizing PEG-PAMAMs with different PEGylation degrees.

• 出版日期2015-11-1