Decreased intracellular granule movement and glucagon secretion in pancreatic alpha cells attached to superior cervical ganglion neurites

作者：Watabe Kiyoto; Yokawa Satoru; Inoh Yoshikazu; Suzuki Takahiro; Furuno Tadahide^*

来源：Molecular and Cellular Biochemistry, 2018, 446(1-2): 83-89.

DOI：10.1007/s11010-018-3275-2

• Pancreatic alpha cell
• Granule movement
• Glucagon secretion
• Neurite
• Spinning disk microscopy

Autonomic neurons innervate pancreatic islets of Langerhans and participate in the maintenance of blood glucose concentrations by controlling hormone levels through attachment with islet cells. We previously found that stimulated superior cervical ganglia (SCG) could induce Ca2+ oscillation in alpha cells via neuropeptide substance P using an in vitro co-culture model. In this study, we studied the effect of SCG neurite adhesion on intracellular secretory granule movement and glucagon secretion in alpha cells stimulated by low glucose concentration. Spinning disk microscopic analysis revealed that the mean velocity of intracellular granules was significantly lower in alpha cells attached to SCG neurites than that in those without neurites under low (2 mM), middle (10 mM), and high (20 mM) glucose concentrations. Stimulation by a low (2 mM) glucose concentration significantly increased glucagon secretion in alpha cells lacking neurites but not in those bound to neurites. These results suggest that adhesion to SCG neurites decreases low glucose-induced glucagon secretion in pancreatic alpha cells by attenuating intracellular granule movement activity.

• 出版日期2018-9