A novel enzyme-free sandwich-like electrochemical immunosensor for the detection of carbohydrate antigen 15-3 based on hierarchical AuPd nanochain networks

作者：Liu, Yan; Weng, Xuexiang; Wang, Ke-Ke; Xue, Yadong; Wang, Ai-Jun; Wu, Liang; Feng, Jiu-Ju^*

来源：Sensors and Actuators B: Chemical , 2017, 247: 349-356.

DOI：10.1016/j.snb.2017.03.024

• AuPd nanochain networks
• Theophylline-7-acetic acid
• Sandwich-like immunosensor
• Carbohydrate antigen 15-3

A novel ultra-sensitive enzyme-free sandwich-like electrochemical immunosensor was fabricated based on hierarchical AuPd nanochain networks (NCNB). The architectures were firstly synthesized by a single-step seedless aqueous method with theophylline-7-acetic acid (T7AA) as a new capping agent. The obtained AuPd NCNB were used for the antibody immobilization and signal amplification. The immunosensor for carbohydrate antigen 15-3 had a good linearity within the concentration range from 0.001 pgmL(-1) to 100 ngmL(-1), with the low detection limit of 0.35 fg mL(-1) (S/N = 3). It showed good reproducibility, high sensitivity and stability, which provided a promising platform for the clinical immunoassay of carbohydrate antigen 15-3.

• 出版日期2017-8
• 单位浙江师范大学