科研之友
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摘要
Microfluidic vascular models were utilized as a novel platform of biochemical analysis. Cell based vascular models were developed by culturing vascular cells in microchannels. A palmtop-sized microfluidic cell culture system, consisting of a microfluidic device and a miniaturized infusion pump, was developed. The proliferation speed and protein expression of human endothelial cells cultured in the system were the same as cells in a culture flask. A microfluidic model of microcirculation containing both blood and lymphatic vessels was developed to examine the vascular permeability. The model was applied to the assay of habu snake venom, and the assay time was reduced from 24 h to 30 min. On the other hand, cell free microfluidic vascular models were developed in order to precisely understand the mechanism of microcirculation. The permeation of nanoparticles through artificial vascular walls was evaluated. The permeation of nanoparticles through interstitium surrounding the blood vessels was also evaluated under cell-free conditions. These models can bridge in vivo analysis and in vitro analysis.
- 出版日期2016-5
