Adipogenesis inhibitory effects of Limonium tetragonum in mouse bone marrow stromal D1 cells

作者：Kwon Myeong Sook; Karadeniz Fatih; Kim Jung Ae; Seo Youngwan; Kong Chang Suk^*

来源：Food Science and Biotechnology, 2016, 25(S1): 125-131.

DOI：10.1007/s10068-016-0109-4

• adipogenesis
• bone marrow
• D1 cell
• Limonium tetragonum
• PPAR gamma

Unbalanced differentiation of bone marrow cells into adipocytes rather than osteoblasts causes severe bone-related disorders, such as osteoporosis. Natural products that can act against unbalanced differentiation are targeted for development of functional dietary supplements. Limonium tetragonum, a halophyte known for anti-oxidant constituents, was studied for beneficial effects in prevention of adipogenesis of bone marrow stromal D1 cells in vitro. Solvent-partitioned extract fractions of L. tetragonum prevented differentiating D1 cells from accumulating lipids. Expression of the key adipogenic markers PPAR gamma, SREBP1c, and C/EBP alpha were inhibited at mRNA and protein levels. Suppressed protein levels of the phosphorylated MAPK-related factors ERK, JNK, and p-38 indicated inhibition of PPAR gamma pathway-linked adipogenesis in D1 cells. The n-BuOH fraction was the most effective fraction for bioactive substances. L. tetragonum inhibited adipogenesis in D1 bone marrow stromal cells due to the presence of bioactive flavonoids.

• 出版日期2016-3