科研之友
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摘要
To determine the virulence factors of Mexican wild-type strains of Campylobacter jejuni and Campylobacter colt, 31 wildtype strains were isolated from food and from humans. The production of cytolethal distending toxin and the adherence and invasion capabilities of these strains were assayed in Vero cells. Hard agar plugs with repellents and attractants were used to examine chemotaxis. Mueller-Hinton agar with supplements was used for motility analysis and to measure hemolytic activity. Nine strains of C. jejuni and eight strains of C. coil exhibited motility, most within a diameter of 2 to 13 mm. Most of the strains reacted to the repellent compounds analyzed, and alpha- and beta-like hemolysis and cytotoxicity in Vero cells were observed for all strains. Isolates adhered to and invaded Vero cells to various degrees. Although strains of C. jejuni exhibited stronger adherence but less invasion compared with strains of C. coil, the difference was not significant (P > 0.05). The strains of C. jejuni and C. coil isolated from food and from patients in Mexico could have major impacts on public health.
- 出版日期2010-11
