Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection

作者：Mukherjee Shankar; Nagajyothi Fnu; Mukhopadhyay Aparna; Machado Fabiana S; Belbin Thomas J; de Carvalho Antonio Campos; Guan Fangxia; Albanese Chris; Jelicks Linda A; Lisanti Michael P; Silva Joao S; Spray David C; Weiss Louis M; Tanowitz Herbert B^*

来源：Genomics, 2008, 91(5): 423-432.

DOI：10.1016/j.ygeno.2008.01.008

• gene array analysis
• infection/inflammation
• cardiomyopathy
• Chagas disease
• Trypanosonia cruzi
• cyclins
• Fas

Chagas disease, characterized by acute myocarditis and chronic cardiomyopathy, is caused by infection with the protozoan parasite Trypanosoma cruzi. We sought to identify genes altered during the development of parasite-induced cardiomyopathy. Microarrays containing 27,400 sequence-verified mouse cDNAs were used to analyze global gene expression changes in the myocardium of a murine model of chagasic cardiomyopathy. Changes in gene expression were determined as the acute stage of infection developed into the chronic stage. This analysis was performed on the hearts of male CD-1 mice infected with trypomastigotes of T. cruzi (Brazil strain). At each interval we compared infected and uninfected mice and confirmed the microarray data with dye reversal. We identified eight distinct categories of mRNAs that were differentially regulated during infection and identified dysregulation of several key genes. These data may provide insight into the pathogenesis of chagasic cardiomyopathy and provide new targets for intervention.

• 出版日期2008-5
• 单位郑州大学