Xenotransplantation of human unrestricted somatic stem cells in a pig model of acute myocardial infarction

作者：Gahremanpour Amir; Vela Deborah; Zheng Yi; Silva Guilherme V; Fodor William; Cardoso Cristiano O; Baimbridge Fred; Fernandes Marlos R; Buja L Maximilian; Perin Emerson C^*

来源：Xenotransplantation, 2013, 20(2): 110-122.

DOI：10.1111/xen.12026

• cord blood stem cell transplantation
• heterologous
• myocardial infarction
• pluripotent stem cells
• transplantation

Background Stem cell therapy may help restore cardiac function after acute myocardial infarction (AMI), but the optimal therapeutic cell type has not been identified. Methods We examined the effects of CD34-/CD45- human unrestricted somatic stem cells (USSCs) in pigs (n=30) with an AMI created by a 90-min occlusion of the left anterior descending coronary artery. Pigs were randomly assigned to receive either USSCs (302 +/- 23x106 cells) or phosphate-buffered saline via 15 NOGA-guided transendocardial injections 10days after AMI. Cyclosporine A (10mg/kg orally, twice a day) was started in all pigs 3days before control or cell treatment. Cardiac function was assessed by echocardiography before injection and at 4 and 8weeks after treatment. Serum titers for pig IgG antibodies against USSCs were also measured at these time points and before AMI. Results Compared with control pigs, USSC-treated pigs showed no significant differences in any of the functional parameters examined. USSC-treated pigs showed variable increases in anti-USSC IgG antibody titers in the blood and chronic inflammatory infiltrates at the cell injection sites. Immunohistochemical studies of the injection sites using human anti-mitochondrial antibodies failed to detect implanted USSCs. Conclusions We conclude that human USSCs did not improve cardiac function in a pig model of AMI. Cell transplantation in a xenogeneic setting may obscure the benefits of stem cell therapy.

• 出版日期2013-4