Application of nanoring amino-functionalized magnetic polymer dispersive micro-solid-phase extraction and ultra fast liquid chromatography-tandem mass spectrometry in dicyandiamide residue analysis of powdered milk

作者：Chen Xiao Hong; Zhou Li Xin; Zhao Yong Gang; Pan Sheng Dong; Jin Mi Cong^*

来源：Talanta, 2014, 119: 187-192.

DOI：10.1016/j.talanta.2013.10.003

• Ultra-fast liquid chromatography-tandem quadrupole mass spectrometry (UFLC-MS/MS)
• Core-shell nanoring amino-functionalized magnetic polymer (CS-NR-MP)
• Dispersive micro-solid-phase extraction (d-mu-SPE)
• Matrix effect
• Dicyandiamide (DCD)
• Powdered milk

In this study, a rapid and accurate ultra-fast liquid chromatography-tandem quadrupole mass spectrometry (UFLC-MS/MS) method combined with dispersive micro-solid-phase extraction (d-mu-SPE) using a core-shell nanoring amino-functionalized magnetic polymer (CS-NR-MP) was established and validated to determine trace dicyandiamide (DCD) in powdered milk. The developed d-mu-SPE cleanup procedure can dramatically reduce the matrix in samples, and lead to a significant reduction in absolute matrix effects. Chromatographic separation was performed on an Acquity UPLC BEH Amide column by using water-acetonitrile (9:91, v/v) as the mobile phase within 2 min. DCD was quantitatively analyzed by using DCD-(N2C2)-N-15-C-13 as an internal standard. The results showed that the recoveries were between 99.8 and 105.6% with RSDs in the range of 0.5-4.9%. The target compound had good linearity in the range of 0.1-20.0 mu g kg(-1) with a correlation coefficient (r) of 0.9996. The limit of quantification (LOQ) was 0.06 mu g kg(-1). This method can be used for the rapid and sensitive determination of ultratrace DCD residue in powdered milk samples.

• 出版日期2014-2-15
• 单位宁波大学