科研之友
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摘要
Hypochlorite has cytotoxic effect and can provoke the development of a number of chronic inflammatory diseases. A new assay for the assessment of hypochlorite scavenging capacity (HSC), based on the differential pulse voltammetric (DPV) analysis of hypochlorite before and after reduction by dietary antioxidants, was developed. This electroanalytical assay was validated by testing linearity, precision, and accuracy. The calibration curves of antioxidant compounds covering polyphenols, ascorbic acid, and glutathione were investigated, and their response sensitivities and linear concentration ranges were determined. The calibration graph of Trolox (reference compound) was linear with a slope of 6.82x10(-3) A L mol(-1). The limit of detection and limit of quantification for Trolox were 2.55 and 8.50 mu M, respectively. Moreover, this method was successfully applied to evaluate the HSC of fruit juices with turbid and colored components. In comparison of popular probe-based methods and hypochlorite scavenging efficacy assay, the proposed DPV method is rapid and better represents the direct scavenging activity of antioxidant samples toward hypochlorite (i.e., the total amount of hypochlorite scavenged by antioxidants). Finally, the chemical meaning of HSC value based on multiple oxidation process (MOS) was analyzed by structural theoretical calculation. It was demonstrated that the proposed DPV-based method is applicable in reflecting the MOS of antioxidants. Overall, the recommended DPV method is simple and enables the direct evaluation of the total hypochlorite scavenging capacity of dietary antioxidants.
