Analysis of the ergosterol biosynthesis pathway cloning, molecular characterization and phylogeny of lanosterol 14 a-demethylase (ERG11) gene of Moniliophthora perniciosa

作者：Ceita Geruza de Oliveira; Vilas Boas Laurival Antonio; Castilho Marcelo Santos; Carazzolle Marcelo Falsarella; Pirovani Carlos Priminho; Selbach Schnadelbach Alessandra; Gramacho Karina Peres; Pereira Ramos Pablo Ivan; Barbosa Luciana Veiga; Guimaraes Pereira Gonalo Amarante; Goes Neto Aristoteles^*

来源：Genetics and Molecular Biology, 2014, 37(4): 683-693.

• Basidiomycota
• fungus
• ergosterol
• Theobroma cacao

The phytopathogenic fungus Moniliophthora pemiciosa (Stahel) Aime & Philips-Mora, causal agent of witches' broom disease of cocoa, causes countless damage to cocoa production in Brazil. Molecular studies have attempted to identify genes that play important roles in fungal survival and virulence. In this study, sequences deposited in the M. perniciosa Genome Sequencing Project database were analyzed to identify potential biological targets. For the first time, the ergosterol biosynthetic pathway in M. pemiciosa was studied and the lanosterol 14 alpha-demethylase gene (ERG11) that encodes the main enzyme of this pathway and is a target for fungicides was cloned, characterized molecularly and its phylogeny analyzed. ERG11 genomic DNA and cDNA were characterized and sequence analysis of the ERG11 protein identified highly conserved domains typical of this enzyme, such as SRS1, SRS4, EXXR and the heme-binding region (HBR). Comparison of the protein sequences and phylogenetic analysis revealed that the M. pemiciosa enzyme was most closely related to that of Coprinopsis cinerea.

• 出版日期2014