HYPERSPECTRAL AUTOFLUORESCENCE IMAGING OF DRUSEN AND RETINAL PIGMENT EPITHELIUM IN DONOR EYES WITH AGE-RELATED MACULAR DEGENERATION

作者：Tong Yuehong; Ben Ami Tal; Hong Sungmin; Heintzmann Rainer; Gerig Guido; Ablonczy Zsolt; Curcio Christine A; Ach Thomas; Smith R Theodore^*

来源：Retina-The Journal of Retinal and Vitreous Diseases, 2016, 36(12): S127-S136.

DOI：10.1097/iae.0000000000001325

• age-related macular degeneration
• autofluorescence
• Bruch membrane
• drusen
• fluorophores
• hyperspectral imaging
• lipofuscin
• nonnegative tensor factorization
• retinal pigment epithelium
• sub-retinal pigment epithelium deposits

Purpose: To elucidate the molecular pathogenesis of age-related macular degeneration (AMD) and interpretation of fundus autofluorescence imaging, the authors identified spectral autofluorescence characteristics of drusen and retinal pigment epithelium (RPE) in donor eyes with AMD.
Methods: Macular RPE/Bruch membrane flat mounts were prepared from 5 donor eyes with AMD. In 12 locations (1-3 per eye), hyperspectral autofluorescence images in 10-nm-wavelength steps were acquired at 2 excitation wavelengths (lambda(ex) 436, 480 nm). A nonnegative tensor factorization algorithm was used to recover 5 abundant emission spectra and their corresponding spatial localizations.
Results: At lambda(ex) 436 nm, the authors consistently localized a novel spectrum (SDr) with a peak emission near 510 nm in drusen and sub-RPE deposits. Abundant emission spectra seen previously (S0 in Bruch membrane and S1, S2, and S3 in RPE lipofuscin/melanolipofuscin, respectively) also appeared in AMD eyes, with the same shapes and peak wavelengths as in normal tissue. Lipofuscin/melanolipofuscin spectra localizations in AMD eyes varied widely in their overlap with drusen, ranging from none to complete.
Conclusion: An emission spectrum peaking at similar to 510 nm (lambda(ex) 436 nm) appears to be sensitive and specific for drusen and sub-RPE deposits. One or more abundant spectra from RPE organelles exhibit characteristic relationships with drusen.

• 出版日期2016-12