Analytical Enantioseparation of β-Substituted-2-Phenylpropionic Acids by High-Performance Liquid Chromatography with Hydroxypropyl-β-Cyclodextrin as Chiral Mobile Phase Additive

作者：Tong, Shengqiang^*; Zhang, Hu; Yan, Jizhong

来源：Journal of Chromatographic Science, 2016, 54(4): 593-597.

DOI：10.1093/chromsci/bmv192

• STATIONARY PHASES
• CAPILLARY ELECTROCHROMATOGRAPHY
• ENANTIOMERIC SEPARATION
• MANDELIC-ACID
• DERIVATIVES
• SELECTOR
• ELECTROPHORESIS
• RESOLUTION

Analytical enantioseparation of five beta-substituted-2-phenylpropionic acids by high-performance liquid chromatography with hydroxypropyl-beta-cyclodextrin (HP-beta-CD) as chiral mobile phase additive was established in this paper, and chromatographic retention mechanism was studied. The effects of various factors such as the organic modifier, different ODS C-18 columns and concentration of HP-beta CD were investigated. The chiral mobile phase was composed of methanol or acetonitrile and 0.5% triethylamine acetate buffer at pH 3.0 added with 25 mmol L-1 of HP-beta-CD, and baseline separations could be reached for all racemates. As for chromatographic retention mechanism, it was found that there was a negative correlation between the concentration of HP-beta-CD in mobile phase and the retention factor under constant pH value and column temperature.

• 出版日期2016-4
• 单位浙江工业大学