科研之友
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摘要
Auger-electron-emitting radioisotopes such as I-125 produce DNA strand breaks within nanometer range of the decay site. Here we analyze these breaks in order to study changes in DNA conformation upon binding with cyclic AMP receptor protein (CRP) in solution. The clear difference we found in break frequency in the CRP-DNA complex, as compared to the naked DNA duplex, correlates with the increased distances between the deoxyriboses and the radioiodine atom caused by the CRP-induced kink observed in the cocrystal. Thus, we demonstrate that I-125 radioprobing can be used to study fine conformational changes of DNA within DNA-protein complexes.
- 出版日期1999-8
