Activation of Ca2+-activated Cl- channel ANO1 by localized Ca2+ signals

作者：Jin Xin; Shah Sihab; Du Xiaona; Zhang Hailin; Gamper Nikita^*

来源：The Journal of Physiology, 2016, 594(1): 19-30.

DOI：10.1113/jphysiol.2014.275107

Ca2+-activated chloride channels (CaCCs) regulate numerous physiological processes including epithelial transport, smooth muscle contraction and sensory processing. Anoctamin-1 (ANO1, TMEM16A) is a principal CaCC subunit in many cell types, yet our understanding of the mechanisms of ANO1 activation and regulation are only beginning to emerge. Ca2+ sensitivity of ANO1 is rather low and at negative membrane potentials the channel requires several micromoles of intracellular Ca2+ for activation. However, global Ca2+ levels in cells rarely reach such levels and, therefore, there must be mechanisms that focus intracellular Ca2+ transients towards the ANO1 channels. Recent findings indeed indicate that ANO1 channels often co-localize with sources of intracellular Ca2+ signals. Interestingly, it appears that in many cell types ANO1 is particularly tightly coupled to the Ca2+ release sites of the intracellular Ca2+ stores. Such preferential coupling may represent a general mechanism of ANO1 activation in native tissues.

• 出版日期2016-1-1
• 单位河北医科大学