Analysis of Monoterpenoid Indole Alkaloids Using Electrospray Ionization Tandem Mass Spectrometry

作者：Li, Li-Mei^*; Li, Guo-You; Fang, Dong-Mei; Wu, Zhi-Jun; Zhang, Guo-Lin

来源：Chemistry of Natural Compounds, 2015, 51(1): 116-120.

DOI：10.1007/s10600-015-1215-x

• monoterpenoid indole alkaloids
• electrospray
• radical ions
• ESI-QTOF
• Winchia calophylla

Three representative monoterpenoid indole alkaloids isolated from the trunk barks of Winchia calophylla were investigated using electrospray quadrupole time-of-flight tandem mass spectrometry (ESI-QTOF-MS/MS) in positive-ion mode. The major CID-fragmentation patterns are the neutral losses of H2O, CH3NH2 (C2H5N or C3H7N), CH2O, HCOOCH3 (HCOOH or CO2), and C4H6 molecules and CH3 radical. High-abundance radical ions at m/z 219, 217, and 205 are formed from corresponding precursor ions by loss of a CH3 radical. The product ion at m/z 168, 144, and 130 in the low-mass range are the characteristic ions of the indole part. A pair of isomers was distinguished unambiguously based on MS/MS spectra. The proposed fragmentation pathways were further supported by ESI-MS/MS/MS and D-labeling experiments.

• 出版日期2015-1
• 单位中国科学院; 中国科学院成都生物研究所; 成都医学院