Anti-inflammatory properties of an extract of M. ilicifolia in the human intestinal epithelial Caco-2 cell line

作者：Wonfor Ruth; Natoli Manuela; Parveen Ifat; Beckman Manfred; Nash Robert; Nash Deborah^*

来源：Journal of Ethnopharmacology, 2017, 209: 283-287.

DOI：10.1016/j.jep.2017.08.006

• Inflammation
• Caco-2 cells
• M. ilicifolia
• Toxicity
• Anti-inflammatory

Ethnopharmacology relevance: Maytenus ilicifolia is a Celastracea plant used in traditional medicine to alleviate digestive tract inflammatory disorders.
Aim of the study: We investigated anti-inflammatory properties of M. ilicifolia crude extract towards Caco-2 cell line, as a model of Toll-like Receptor 2 (TLR-2) inflammatory pathway.
Materials and methods: Toxicity was assessed following culture of Caco-2 with M. ilicifolia, using apparent cell permeability and trans-epithelial electric resistance. Anti-inflammatory properties of M. ilicifolia were assessed through IL-8 secretion and TLR-2 associated gene expression of Caco-2 cells with or without an LTA challenge.
Results: M. ilicifolia was not toxic to Caco-2 cells. M. ilicifolia down-regulated TLR2 expression with and without LTA challenge but had no effect on other genes. Following LTA challenge of Caco-2 cells, 100 and 200 mu g/mL M. ilicifolia abrogated IL-8 secretion.
Conclusions: We provide preliminary data for some M. ilicifolia anti-inflammatory properties. Further research must establish the full extent and mode of action on particular inflammatory pathways.

• 出版日期2017-9-14