Demineralized Bone Matrix Promotes Bone Regeneration In Vivo: A Canine Study

作者：Liu, Guolin; Wang, Lingxiao; Hu, Lei; Liu, Changying; Jin, Luyuan; Shan, Zhaochen; Gao, Zhenhua^*

来源：Journal of Biomaterials and Tissue Engineering, 2018, 8(5): 716-722.

DOI：10.1166/jbt.2018.1796

• Demineralized Bone Matrix
• Bone Marrow Mesenchymal Stem Cells
• Biomaterial
• Osteogenesis
• Differentiation

The aim of the present study was to investigate the effect of demineralized bone matrix (DBM) on bone marrow mesenchymal stem cells (BMSCs) mediated tissue engineering-bone formation in vivo. BMSCs derived from canine bone marrow were isolated and cultured in vitro. The osteogenic and adipocytic differentiation of BMSCs were assessed by Alizarin Red staining and Oil Red O staining. Adhesion of BMSCs on the DBM was observed using scanning electron microscopy. The DBM seeded with BMSCs were implanted under one side of the latissimus dorsi muscle of dogs as experimental group, while the DBM without BMSCs were implanted under the other side of latissimus dorsi muscle as the controls. X-ray observation showed the experimental group had a high mineral density compared to the control group. Histology examination revealed that the new bone was regenerated in the experimental group, while there was only connecting tissue formed in the control group. Taken together, these results indicate that DBM could support bone regeneration combined with BMSCs and be developed as a potential bioscaffold for future bone regeneration.

• 出版日期2018-5
• 单位首都医科大学