Antifungal activity of the essential oil of Zanthoxylum bungeanum and its major constituent on Fusarium sulphureum and dry rot of potato tubers

作者：Li Xing-dong^*; Xue Hua-li

来源：Phytoparasitica, 2014, 42(4): 509-517.

DOI：10.1007/s12600-014-0388-3

• alpha-pinene
• Dry rot
• Zanthoxylum oil

The composition of the essential oil of Zanthoxylum bungeanum was analyzed by gas chromatography-mass spectrometry (GC-MS). A total of 35 components, which constitute 99.10% of the oil, were identified. The major constituent was alpha-pinene (19.59%). Minimum inhibitory concentrations (MICs) of the essential oil and alpha-pinene against Fusarium sulphureum were determined to be 6.25% and 12.50%, respectively. A determination of the antifungal activity and inhibitory effect of the essential oil and alpha-pinene showed that the essential oil more significantly decreased cell membrane integrity and inhibited spore germination than alpha-pinene. Observations using scaning electron microscope revealed morphological modification including mycelium asymmetry, hyphal swelling, curling, and a secretory appearance. Ultrastructural alterations were also observed including cell empty cavity and osmiophilic granule generation. In vivo testing showed that the oil and alpha-pinene effectively controlled dry rot of potato inoculated with F. sulphureum. These findings indicated that the oil and alpha-pinene directly inhibit fungal growth, and the essential oil showed pronounced potential than alpha-pinene.

• 出版日期2014-9
• 单位兰州交通大学; 甘肃农业大学