Development of In Situ Hybridization for the Detection of Mycoplasma hyorhinis in Formalin-Fixed Paraffin-Embedded Tissues from Naturally Infected Pigs with Polyserositis

作者：Kim Bongtae; Lee Kichan; Han Kiwon; Kim Duyeol; Ha Yooncheol; Kim Chung Hyun; Oh Yeonsu; Kang Ikjae; Lee Jeehoon; Chae Chanhee^*

来源：Journal of Veterinary Medical Science, 2010, 72(9): 1225-1227.

• in situ hybridization
• Mycoplasma hyorhinis
• swine

The aim of this study was to develop in situ hybridization for detection of Mycoplasma hyorhinis in formalin-fixed, paraffin-wax-embedded tissues from pigs with polyserositis. M hyorhinis was isolated from the spleen (2 pigs) and pericardium (1 pig). M. hyorhinis DNA was detected 16 out of 20 pigs with polyserositis. In situ hybridization produced a distinct positive signal for the M. hyorhinis p37 gene in inflammatory cells in the polyserositis. In situ hybridization developed in the present study present diagnostic tools capable of detection of M. hyorhinis in formalin-fixed, paraffin-wax-embedded tissues from the naturally infected pigs.

• 出版日期2010-9