Direct determination of phenolic acids and hydroxymethylfurfural in wines elaborated in Vale do Sao Francisco region-Brazil by HPLC DAD

作者：Dias Fabio de Souza^*; Palma Lovillo Miguel; Barroso Carmelo G; Fagundes Carneiro Angelica; David Jorge Mauricio

来源：Analytical Methods, 2017, 9(4): 643-648.

DOI：10.1039/c6ay02657e

The present paper describes a direct method for the determination of gallic acid, hydroxymethylfurfural, vanillic, caffeic, p-coumaric, ferulic, caftaric, cis-coutaric, fertaric and trans-coutaric acids in Brazilian red wines by direct injection employing reverse phase high performance liquid chromatography (RP HPLC DAD). The method was performed using a sample volume of 10 mu L and the separation process was established employing a monolithic column. The method was applied for analysis of red wine samples collected from the Sao Francisco region, Bahia State, Brazil. Ten wine samples were analysed and the contents of caftaric acid varied from 13.28 to 46.83 mg L-1, cis-coutaric acid from 1.01 to 2.13 mg L-1, trans-coutaric acid from 2.13 to 17.56 mg L-1, fertaric acid from 1.11 to 2.43 mg L-1, ferulic acid from 0.31 to 3.97 mg L-1, hydroxymethylfurfural from 0.59 to 8.83 mg L-1, vanillic acid from 2.90 to 11.57 mg L-1, caffeic acid from 4.20 to 14.20 mg L-1, p-coumaric acid from 0.26 to 12.46 mg L-1 and ferulic acid from 0.31 to 3.97 mg L-1.

• 出版日期2017