New microsatellite loci isolated via next-generation sequencing for two endangered pronghorn from the Sonoran Desert

作者：Munguia Vega Adrian^*; Klimova Anastasia; Culver Melanie

来源：Conservation Genetics Resources, 2013, 5(1): 125-127.

DOI：10.1007/s12686-012-9749-8

• Antilocapra americana
• Sonoran desert
• Microsatellites
• Ungulates

We isolated 16 novel microsatellite loci in two subspecies of endangered desert pronghorns (Antilocapra americana sonoriensis and Antilocapra americana peninsularis) using a shotgun pyrosequencing approach. All and 87.5 % of the loci were polymorphic within each subspecies, respectively. The mean number of alleles per locus was 4.86 (range 2-8) and 2.5 alleles per locus (range 1-4 alleles), and observed heterozygosity ranged from 0.13 to 0.78 (mean 0.48) and 0.00 to 0.61 (mean 0.31), respectively. We did not find significant linkage disequilibrium among loci pairs and only one locus deviated significantly from Hardy-Weinberg equilibrium in peninsularis.

• 出版日期2013-3