Multidrug resistance genes, including bla(KPC) and bla(CTX-M-2 %26apos;) among Klebsiella pneumoniae isolated in Recife, Brazil

作者：Cabral Adriane Borges; de Andrade Melo Rita de Cassia; Vieira Maciel Maria Amelia; Souza Lopes Ana Catarina

来源：Revista da Sociedade Brasileira de Medicina Tropical, 2012, 45(5): 572-578.

DOI：10.1590/S0037-86822012000500007

Introduction: The prevalence of cephalosporins and carbapenem-resistant Klebsiella pneumoniae strains is rising in Brazil, with potential serious consequences in terms of patients%26apos; outcomes and general care. Methods: This study characterized 24 clinical isolates of K. pneumoniae from two hospitals in Recife, Brazil, through the antimicrobial susceptibility profile, analyses of beta-lactamase genes (bla(TEM), bla(SHV), bla(CTX-M%26apos;), bla(KPC), bla(VIM), bla(IMP), and bla(SPM)), plasmidial profile and ERIC-PCR (Enterobacterial repetitive intergenic consensus-polymerase chain reaction). Results: ERIC-PCR and plasmidial analysis grouped the isolates in 17 and 19 patterns, respectively. Six isolates from one hospital presented the same pattern by ERIC-PCR, indicating clonal dissemination. All isolates presented bla(SHV), 62.5% presented bla(CTX-M-2%26apos;), 29% bla(TEM), and 41.7% bla(KPC). Metallo-beta-lactamase genes bla(VIM), bla(IMP), and bla(SPM) were not detected. Eleven isolates were identified carrying at least 3 beta-lactamase studied genes, and 2 isolates carried bla(SHV), bla(TEM), bla(CTX-M-2%26apos;), and bla(KPC) simultaneously. Conclusions: The accumulation of resistance genes in some strains, observed in this study, imposes limitations in the therapeutic options available for the treatment of infections caused by K. pneumoniae in Recife, Brazil. These results should alert the Brazilian medical authorities to establish rigorous methods for more efficiently control the dissemination of antimicrobial resistance genes in the hospital environment.

• 出版日期2012-10