Proteome Alterations in Primary Human Alveolar Macrophages in Response to Influenza A Virus Infection

作者：Liu Lin; Zhou Jianhong; Wang Yimeng; Mason Robert J; Funk Cornelius Joel^*; Du Yuchun

来源：Journal of Proteome Research, 2012, 11(8): 4091-4101.

DOI：10.1021/pr3001332

• influenza A virus
• IFN-alpha/beta
• quantitative proteomics
• primary alveolar macrophage
• protein expression
• LC-MS/MS
• SILAC

To obtain a global picture of how alveolar macrophages respond to influenza A virus (IAV) infection, we used a quantitative proteomics method to systematically examine protein expression in the IAV-infected primary human alveolar macrophages. Of the 1214 proteins identified, 43 were significantly up-regulated and 63 significantly down-regulated at >95% confidence. The expression of an array of interferon (IFN)-induced proteins was significantly increased in the IAV-infected macrophages. The protein with the greatest expression increase was ISG15, an IFN-induced protein that has been shown to play an important role in antiviral defense. Concomitantly, quantitative real-time PCR analysis revealed that the gene expression of type I IFNs increased substantially following virus infection. Our results are consistent with the notion that type I IFNs play a vital role in the response of human alveolar macrophages to LAV infection. In addition to the IFN-mediated responses, inflammatory response, apoptosis, and redox state rebalancing appeared also to be major pathways that were affected by IAV infection. Furthermore, our data suggest that alveolar macrophages may play a crucial role in regenerating alveolar epithelium during IAV infection.

• 出版日期2012-8